Cloning of an epithelial chloride channel from bovine trachea.
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چکیده
منابع مشابه
Reconstitution of an epithelial chloride channel. Conservation of the channel from mudpuppy to man
We have previously shown that monoclonal antibody E12 (MAb E12), one of several such antibodies raised against theophylline-treated Necturus gallbladder (NGB) epithelial cells, inhibits the chloride conductance in the apical membrane of that tissue. Since chloride channels are critical to the secretory function of epithelia in many different animals, we have used this antibody to determine whet...
متن کاملIndependently gated multiple substates of an epithelial chloride-channel protein.
We have purified a protein from Necturus maculosus gallbladder cells that forms chloride channels in an artificial membrane. The same protein apparently can form channels that are highly selective for chloride but can have conductances varying from 9 to about 150 pS. The high-conductance channels are blocked by the monoclonal antibody used to purify the protein, but this antibody has no effect ...
متن کاملEpithelial Chloride Channel Development of lnhibitory Ligands
I N T R O D U C T I O N Chloride channels are present in the plasma membranes o f epithelia (Frizzell et al., 1981), nerve (Hamill et al., 1983), and muscle (Blatz and Magleby, 1985), as well as in intraceilular membranes such as Golgi apparatus (Glickman et al., Address reprint requests to Dr. Qais AI-Awqati, Depts. of Medicine and Physiology, College of Physicians and Surgeons, Columbia Unive...
متن کاملEpithelial chloride channel. Development of inhibitory ligands
Chloride channels are present in the majority of epithelial cells, where they mediate absorption or secretion of NaCl. Although the absorptive and secretory channels are well characterized in terms of their electrophysiological behavior, there is a lack of pharmacological ligands that can aid us in further functional and eventually molecular characterization. To obtain such ligands, we prepared...
متن کاملExtracellular chloride regulates the epithelial sodium channel.
The extracellular domain of the epithelial sodium channel ENaC is exposed to a wide range of Cl(-) concentrations in the kidney and in other epithelia. We tested whether Cl(-) alters ENaC activity. In Xenopus oocytes expressing human ENaC, replacement of Cl(-) with SO4(2-), H2PO4(-), or SCN(-) produced a large increase in ENaC current, indicating that extracellular Cl(-) inhibits ENaC. Extracel...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1996
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)45100-3